A: 3X Firefly Assay Buffer
Stock Solutions (store at -80 °C)	Volume per 100 ml 3X Firefly Assay Buffer	Final Concentration (3X)
500 mM DTT	3.0 ml	15 mM
10 mM coenzyme A	6.0 ml	0.6 mM
100 mM ATP	0.45 ml	0.45 mM
80 mg/mlluciferin	0.525 ml	4.2 mg/ml
Triton lysis buffer	90.025 ml	--
B. Triton Lysis Buffer
Component (store at RT)	Volume per 100 ml Triton Lysis Buffer	Final Concentration
Tris-HCl powder	1.705 g	0.1082 M
Tris-base powder	0.508 g	0.0419 M
5 M NaCl	1.50 ml	75 mM
1 M MgCl2	0.30 ml	3 mM
Triton X-100 pure liquid	0.75 ml	0.25%
Water	to 100 ml total volume	--
C. 3X Renilla Assay Buffer
Stock Solutions (store at -80 °C)	Volume per ~100 ml 3X Renilla Assay Buffer	Final Concentration (3X)
10 mM PTC124 in DMSO	0.6 ml	0.06 mM
2 mM h-CTZ in ethanol	0.5 ml	0.01 mM
Renilla Salts	100 ml	--
D. Renilla Salts
Component (store at RT)	Volume per 100 ml Renilla Salts	Final Concentration
0.5 M Na2EDTA	9.0 ml	45 mM
Na Pyrophosphate	1.34 g	30 mM
NaCl	8.33 g	1.425 M
Water	to 100 ml total volume	--