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. 2014 Jan 26;2(1):2. doi: 10.1002/prp2.18

Figure 1.

Figure 1

Effect of NJ on the transcriptional activity of human constitutive androstane receptor (hCAR). (A) Chemical structures of NJ, NC, and NH. (B) HepG2 cells were transfected with the pG5luc reporter plasmid (0.1 μg) together with the expression vectors for GAL4/DBD (0.05 μg) or GAL4/DBD-hCAR/LBD (0.05 μg) and pGL4.74 (0.01 μg) plasmids. Cells were treated with NJ, NC, and NH (10 μmol/L) or a positive control [PK11195 (10 μmol/L)]. After 24 h, luciferase activity was measured using the Dual-Luciferase Reporter Assay System. Results are shown as fold activation over the solvent control of GAL4/DBD empty plasmid (mean ± SD, n = 3).