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. 2014 Jan 26;2(1):2. doi: 10.1002/prp2.18

Figure 3.

Figure 3

Effects of NJ on the expression of endogenous CAR target genes in HepTR/human constitutive androstane receptor (hCAR) cells. (A) HepTR/hCAR cells were transfected with the PBREM-luc and pGL4.74 plasmids. The transfected cells were treated with increasing concentrations of NJ (0.1, 1, 5, 10, 25 μmol/L) and solvent control (0.1% DMSO) in the absence or presence of Tet. After 24 h, luciferase activity was measured using the Dual-Luciferase Reporter Assay System. Results are shown as fold activation over the solvent control (mean ± SD, n = 4); **P < 0.01. (B, C) HepTR/hCAR cells were treated with NJ (5 or 10 μmol/L) or solvent control (0.1% DMSO) in the absence or presence of Tet. After 48 h, cells were harvested and the mRNA level of CYP2B6 (B) and CYP3A4 (C) was measured by real-time qRT-PCR. Results normalized against β-actin mRNA levels are expressed as fold activation over the solvent control (mean ± SD, n = 4); *P < 0.05; **P < 0.01.