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Representative sections of the 1H,13C-HSQC spectra of titrations of CaM with SF. Sevoflurane (SF, A) titrations to methionine methyl region of apo-CaM (B) and (Ca2+)4-CaM (C) in blue, overlaid with spectra taken after successive additions of 2.4 (magenta), 5.0 (purple), and 10 (red) mmol/L SF.
