Figure 3.
Images of enzymatically isolated fibers from flexor digitorum brevis (FDB) muscles after treatment with the glibenclamide (Glib.) and repaglinide (Repa.) at different incubation time. The fiber diameter and morphology were measured at a 10× magnification; mortality was evaluated at a 5× magnification using QuantiCell 900 integrated imaging system. Isolated fibers, before analysis, were equilibrated in DMEM (300 mOsmol L) for 15 min at 25°C. Fiber diameter and mortality following incubation for 1–48 h with DMEM solution used as control and DMEM solution + glibenclamide (10−5 mol/L) and repaglinide (10−5 mol/L). No effects were observed after 1 h incubation time in the presence of the drugs. The glibenclamide and repaglinide treatments reduced the fiber diameter after 24 h of incubation with respect to the controls. The fiber mortality increased with drug treatments with respect to the control after 48 h of incubation time.