Figure 2.

PGT modulates PGE₂-induced Ca²⁺ responses and medium PGE₂ levels. (A) Dose-dependent responses of the increase in intracellular Ca²⁺ concentration induced by PGE₂ stimulation of EP₁-HEK (n = 37 cells), and of PGT-EP₁-HEK cells in the absence (n = 211 cells) and presence of the PGT blocker T26A (5 μmol/L, n = 340 cells). PGT shifted the PGE₂/Ca²⁺ dose–response curve to the right; PGT inhibition by T26A reversed this effect (see text for statistical analyses). (B) Final PGE₂ concentration in media following 10 min exposure to EP₁-HEK or PGT-EP₁-HEK monolayers in the presence and absence of T26A (5 μmol/L). PGT expression lowered the medium PGE₂ concentration. Values are mean ± SEM (n = 3 for each experiment). *P < 0.05.