Figure 4.

PGT regulates cAMP accumulation in response to autocrine/paracrine EP₄-mediated PGE₂ signaling. WT-MDCK and PGT-GFP-MDCK cells were transiently transfected with the EP₄ receptor cDNA for 24 h, then incubated for 18 h with 250 μmol/L IBMX, and afterward treated with 10 μmol/L bradykinin in the absence or presence of 5 μmol/L T26A for 30 min. (A) PGE₂ concentrations in the media. (B) Intracellular cAMP accumulation. PGT expression reduced both the concentration of PGE₂ in the medium and the accumulation of intracellular cAMP in response to 30 min exposure to bradykinin. Both of these PGT effects were reversed by the PGT inhibitor T26A. Values are mean ± SEM (n = 3 for each experiment). *P < 0.05.