Figure 5.

Concentration–response curves for the inhibition of 10 μmol/L 5-CT-stimulated cAMP production by MEL-9 and LP-211 in HEK-hu5-HT₇ cells in preincubation/washout experiments. Cells were preincubated in the absence (control) or presence of the compounds at increasing concentrations (ranging from 100 pmol/L to 10 μmol/L) for 30 min. After a compound washout procedure (see Materials and Methods), the cells were stimulated with 10 μmol/L 5-CT for 30 min. Basal values were subtracted from 5-CT-stimulated values and expressed as percent of the control 5-CT response. Data are the mean values ± SEM from three independent experiments performed in triplicate.