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. 2014 Jun 23;2(4):e00052. doi: 10.1002/prp2.52

Figure 7.

Figure 7

Vasorelaxation profile of the putative TRPA1 agonist cinnamaldehyde. Mesenteric arterioles from CD1 mice (A–B) or WT and TRPA1 KO mice (C–F). Vessels were preconstricted with U46619 (10 μmol/L) in order to observe the vasorelaxation response. Concentration-response curves to CA were constructed in vessels (A) intact or denuded or endothelium, (B) in the presence of HC030031 (50 μmo/L) or vehicle (0.05% v/v DMSO), and (C) in WT or TRPA1 KO mesenteric arterioles. (D) illustrates the effects of HC030031 (50 μmol/L) or vehicle (0.05% v/v DMSO) in WT and TRPA1 KO arterioles on CA-induced relaxation, the table below contains the pEC50 and EMax values. (E) shows the effects of CGRP8-37 (3 μmol/L) or vehicle (0.1% v/v of 0.01% BSA in double deionized water) in arterioles collected from WT or TRPA1 KO mice on CGRP-induced relaxation. (F) shows the effects of CGRP8-37 (3 μmol/L) or vehicle in arterioles collected from WT or TRPA1 KO mice on CA responses. N is indicated and data is shown ± SEM. In (D), *P < 0.05 compared to TRPA1 KO vehicle. pEC50 and EMax from (A–C) analyzed through two-tailed unpaired t-test, while (D–F) analyzed by two-way ANOVA with Bonferroni’s post hoc test. In (E), ***P < 0.001 compared to TRPA1 WT vehicle, and ###P < 0.001 compared to TRPA1 KO vehicle.