Figure 2.

Mefloquine induces apoptotic mast cell death. Samples of 0.5 × 10⁶ bone marrow-derived mast cells (BMMCs) (WT, serglycin−/− and mMCP-6−/−) were cultured in the presence or absence of 20 μmol/L of mefloquine. (A) After 8 h incubation, cells were collected and cell death was assessed by Annexin V/PI staining and flow cytometry analysis. The Annexin V⁻/PI⁻ population corresponds to viable cells whereas Annexin V⁺/PI⁻ and Annexin V⁺/PI⁺ populations represent apoptotic and necrotic cells, respectively. (B) Representative dot plots showing Annexin V/PI staining of non- and mefloquine-treated WT, serglycin−/− and mMCP-6−/− mast cells. Data represent means of duplicates ± SEM. **P < 0.01; *P < 0.05. AnnV, Annexin V.