Table 1.
Primer sequences used for the RT-qPCR analysis and conditions
| PCR conditions | ||||
|---|---|---|---|---|
| Gene | Primer sequence (5′ → 3′) | Product size (bp) | Denaturation | Annealing |
| CYP3a11-F | GCC ATT TTT AGG CAC TGT GCT GA | 124 | 95°C, 15 sec | 60°C, 30 sec |
| CYP3a11-R | TGT GAC AGC AAG GAG AGG CGT | |||
| CYP3a13-F | GAC CTG ATC CCA AAC TTT TCC | 211 | 95°C, 15 sec | 60°C, 30 sec |
| CYP3a13-R | TCC TTC TCC TAA TCC CTG CC | |||
| CYP3a25-F | TGG GCA TAC TCA ACT TTC C | 148 | 95°C, 15 sec | 60°C, 30 sec |
| CYP3a25-R | CCA CAA GAA TCA GAA GGT | |||
| CYP3a44-F | CTG AGC TTT CTC AGT GTC TGT G | 102 | 95°C, 15 sec | 60°C, 30 sec |
| CYP3a44-R | GAT CCC ATG AGA AAC GGT GAA G | |||
| Beta-actin-F | TGT TAC CAA CTG GGA CGA CA | 165 | 95°C, 15 sec | 60°C, 30 sec |
| Beta-actin-R | GGG GTG TTG AAG GTC TCA AA | |||
| CAR-F | CTC AAG GAA AGC AGG GTC AG | 168 | 95°C, 15 sec | 60°C, 30 sec |
| CAR-R | GGT CTC CAC ACA CCA CAC AG | |||
| PXR-F | AGG GGG AAA TCC AAC AAA AG | 184 | 95°C, 15 sec | 60°C, 30 sec |
| PXR-R | ACA CGG CAG ATT TGA AGA CC | |||
| RPLP0-F | ATC ACA GAG CAG GCC CTG CA | 96 | 95°C, 15 sec | 57°C, 30 sec |
| RPLP0-R | CAC CGA GGC AAC AGT TGG GT | |||