Figure 3.

Three-dimensional reconstruction showing spatial relationships of DVR (green tubules) and DTLs (red tubules) to CDs (blue tubules) for a single CD cluster. DTL segments that do not express AQP1 are shown in gray. DTLs and DVR lie at the periphery of the central core of CDs, within the intercluster region, and A–D show that this relationship continues along the entire axial length of the CD cluster. Axial positions of A–D are indicated by the curly brackets in the right panel. Tubules are oriented in a corticopapillary direction, with the upper edge of the image near the outer medullary–inner medullary border. The interstitial area within the red boundary line is the “intracluster” region, and the interstitial area between the red and white boundary lines is the “intercluster” region. Scale bar, 500 μm. Reproduced from reference 39 with permission.