Figure 5.

Knockdown of PDE4D inhibits tumor growth of NPC cells in nude mice. (A) CNE2 cells infected with LV-PDE4D shRNA and ctr-shRNA were injected subcutaneously into nude mice. At two weeks post-implantation, the LV-PDE4D shRNA-infected cells produced smaller tumors than those of the ctr-shRNA group. The growth curve represents the tumor volumes. Data are presented as the mean ± standard error of the mean of five mice. (B) At two weeks post-transplantation, the transplanted tumors of the two groups (each of five mice) were sectioned and stained for p-EGFR and p-AKT. Images were captured under inverted microscope at ×200 magnification. ^*P<0.05 and ^**P<0.01. shRNA, short hairpin RNA; p-EGFR, phosphorylated epidermal growth factor receptor; p-AKT, phosphorylated AKT; ctr-shRNA, control shRNA lentiviral particles.