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Demethylation of SOCS1 and p16 genes upon treatment with DNMT1 siRNA. RPMI-8226 cells were grown and transfected with DNMT1 siRNA or negative control oligonucleotides. Genomic DNA from the cells was isolated and subjected to nested methylation-specific polymerase chain reaction assays. All experiments were performed three times. (A) p16 gene. (B) SOCS1 gene. SOCS1,suppressor of cytokine signaling 1; DNMT1, DNA methyltransferase 1; M, methylated; U, unmethylated.
