Table I.

RT-PCR primer sequences.

Primer sequences (5′-3′)	Product length (bp)	Annealing temperature °(C)	Detection temperature (°C)
H3F3A	76	65	72
F: AGGACTTTAAAACAGATCTGCGCTTCCAGAG
R: ACCAGATAGGCCTCACTTGCCTCCTGC
RPLP0	69	65	72
F: ACGGATTACACCTTCCCACTTGCTGAAAAGGTC
R: AGCCACAAAGGCAGATGGATCAGCCAAG
RPS17	87	64	72
F: AAGCGCGTGTGCGAGGAGATCG
R: TCGCTTCATCAGATGCGTGACATAACCTG
MK167	156	56	81
F: TCCTTTGGTGGGCACCTAAGACCTG
R: TGATGGTTGAGGCTGTTCCTTGATG
BAX	137	56	81
F: AGAGGTCTTTTTCCGAGTGGCAGC
R: TTCTGATCAGTTCCGGCACCTTG
BCL2	122	56	81
F: TTGGCCCCCGTTGCTTTTCCTC
R: TCCCACTCGTAGCCCCTCTGCGAC
BIRC5	83	65	72
F: AGTGTTTCTTCTGCTTCAAGGAGCTGGAAG
R: ACCGGACGAATGCTTTTTATGTTCCTCTATG
EGFR	106	58	81
F: AGCTTCTTGCAGCGATACAGCTCAGAC
R: TGGGAACGGACTGGTTTATGTATTCAGG
VEGF	267	60	72
F: TGCTGTAGGAAGCTCATCTC
R: ATCACGAAGTGGTGAAGTTC
CCND1	160	03	86
F: TGTCCTACTACCGCCTCACACGCTTCCTCTCCAG
R: TCCTCTTCCTCCTCCTCGGCGGCCTTG
CCNE1	138	68	68
F: TTCTTGAGCAACACCCTCTTCTGCAGCC
R: TCGCCATATACCGGTCAAAGAAATCTTGTGCC
WWOX
I step	171	63	72
F: TGCAACATCCTCTTCTCCAACGAGCTGCAC
R: TCCCTGTTGCATGGACTTGGTGAAAGGC
II step	150	63	77
F: GAGCTGCACCGTCGCCTCTCCCCAC
R: TCCCTGTTGCATGGACTTGGTGAAAGGC

F, forward; R, reverse.