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. 2014 Sep 5;8(5):2215–2220. doi: 10.3892/ol.2014.2507

Figure 1.

Figure 1

Polymerase chain reaction (PCR)-generated double restriction enzyme sites genotyping assay. The central sequence was the reference genomic DNA sequence. This assay was a nested PCR assay where two primer sets, the outer and inner primers, were used. Each of the inner primers had one base mismatch to the reference sequence for creating a restriction site. The cutting site of BsmAI (GTCTC) was created if the allele type on rs9679162 was G (corresponding to C in the other strand). The cutting site of BspMI (ACCTGC) was created if the allele type on rs6752303 was C.