Table I.
Primer sequence | Tm,°C |
---|---|
Outer primer | |
TCACGAGGCCAACATTCTAG | 58.9 |
TTAGATTCTGCATGGCTCAC | 56.4 |
Inner primer | |
GAAAGCAAAGAGATATATATAACGTCT | 59.2 |
ACCACCTTCCCCTTATTCACCTG | 66.2 |
Tm, temperature.
Primer sequence | Tm,°C |
---|---|
Outer primer | |
TCACGAGGCCAACATTCTAG | 58.9 |
TTAGATTCTGCATGGCTCAC | 56.4 |
Inner primer | |
GAAAGCAAAGAGATATATATAACGTCT | 59.2 |
ACCACCTTCCCCTTATTCACCTG | 66.2 |
Tm, temperature.