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. 2014 Sep 4;8(5):1919–1924. doi: 10.3892/ol.2014.2499

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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DAC inhibits the cell growth of cholangiocarcinoma cell lines. (A) TFK-1 and (B) QBC939 cells were treated with the indicated concentrations of DAC for the indicated time periods. Relative cell growth inhibition was evaluated by the Cell Counting Kit-8 assay. All assays were performed at least in triplicate. The inhibition rate was in comparison with untreated cells. ^*P<0.05 and ^#P<0.01, vs. untreated cells. (C) Clonogenic assay showed the long-term effects of treatment of TFK-1 cells with DAC. TFK-1 cells were treated with the indicated concentrations of DAC for five days. Images of petri dishes from a representative experiment are shown. DAC, decitabine.