Figure 6.

Effect of PLA2R1 knockdown on sPLA₂ expression in PC-3 cells. PLA2R1 expression was inhibited in PC-3 cells using shRNA, and changes in the expression of various sPLA₂ isoforms was determined by immunoblot analysis (A) and qRT-PCR (B). Data in panel A are indicative of at least three separate experiments. Data in panel B are represented as the mean ± SEM of at least three separate experiments (n = 3/study). *Indicates a significant difference (p < 0.05) as compared to cells expressing scrambled shRNA.