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CLSM Z-stack maximum fluorescence intensity projections of osteoblast mineral depositions on (A) non-microcracked and (B) microcracked HA at 21 days. The OBs were pulsed with Xylenol Orange every other day during culturing since day four. The Xylenol Orange fluorescence stain was excited at 543 nm and emission was detected through a BA560-620 emission filter (represented in orange).
