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. Author manuscript; available in PMC: 2014 Oct 6.
Published in final edited form as: Compr Physiol. 2012 Oct;2(4):2733–2752. doi: 10.1002/cphy.c120002

Figure 7. Immunocytochemical distribution of the Mas receptor in the adult sheep kidney and natriuretic influence of Ang-(1-7).

Figure 7

Upper panel: Signal for Mas receptor in proximal tubules (PT) and distal tubules but not glomerulus in renal cortex (A); positive staining of collecting ducts in cortex (B); Mas staining of thick ascending limb of Henle (TAL) and vasa recta (VR) in renal medulla; antigenic peptide for primary antibody abolishes Mas staining in adjacent tissue sections (D-F). Binding of the primary antibody against the Mas receptor protein was followed by the secondary antibody conjugated to Alexa Fluor 488 (green fluorescence) and the nuclear marker stain DAPI (blue). Adapted from Gwathmey et al. (59).

Lower panel: Ang-(1-7) infusion increases sodium excretion (% of an acute sodium load) in control sheep as compared to saline infusion (Vehicle). The natriuretic response to Ang-(1-7) was absent in sheep prenatally exposed to the glucocorticoid betamethasone (Beta). Data are means, n=11-12 sheep. Adapted from Tang et al (142).