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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mutat Res. 2014 Dec 1;770:1–8. doi: 10.1016/j.mrfmmm.2014.08.004

Fig. 3.

Fig. 3

Elevation of MN-γ-H2AX (+) in p400 knockdown cells. U2OS cells and HEK-293 cells were transfected with siRNA duplexes specific to p400 or negative oligo in serum-free medium for 5h, and then replaced with complete medium for 24 h. The expression of p400 mRNA and protein levels in U2OS cells (A) and HEK-293 cells (B) were measured by real-time quantitative RT-PCR assay and Western blotting, respectively. C. The levels of ROS in sip400 U2OS cells and sip400 HEK-293 cells were analyzed by flow cytometry. D. Increased MN-γ-H2AX (+) formation in sip400 U2OS cells and sip400 HEK-293 cells cells. *P<0.05, ** P < 0.01, when compared with each siNeg cells.