Table 1.

Effects of IL-17a, angiotensin II and mechanical stretch on collagen expression by mouse aortic fibroblasts

Treatment	Col 1a1	Col 3a1	Col 5a1	Angiotensin II (100 nM)
				Col 1a1	Col 3a1	Col 5a1
Control	1.00±0.05	1.00±0.34	1.00±0.12	1.03±0.02	1.59±0.08	1.24±0.25
IL-17a (100 ng/mL)	1.20±0.07	4.63±0.66 **	1.89±0.10 *	1.12±0.12	4.28±0.33 **	1.74±0.20
5% Stretch	1.33±0.25	1.98±0.20	1.39±0.21	NA	NA	NA
10% Stretch	2.24±0.24**	3.40±0.53 ** #	2.45±0.37 **	2.80±0.34 **	7.50±1.43 **	2.68±0.62**
IL-17a (100 ng/mL) + 10% Stretch	2.16±0.13* *	3.67±0.41 **	2.32±0.43 **	NA	NA	NA

Primary mouse aortic fibroblasts were exposed to 5% or 10% cyclic stretch or exposed to IL-17a (100 ng/mL) in the presence or absence of angiotensin II (100 nM) for 36 hours. Relative quantity of mRNA was calculated based on the ΔΔCt value normalized to control. Data are expressed as mean ± SE. The effects of IL17a and mechanical stretch are analyzed with one-way ANOVA, while the effect of angiotensin II by two-way ANOVA (n=3–6),

^*p<0.05,

^**p<0.01 vs. control;

^#p<0.05 vs. angiotensin II. NA, not assessed.