Table 1.
Functional classification of the genes whose expression is altered after a 6-h exposure to PACAP
| Function | Clone ID | Avg ± SEM | ERK | PKA | Direct | Gene |
|---|---|---|---|---|---|---|
| Neuritogenesis | ||||||
| C86855 | 5.8 ± 0.7 | yes | no | yes | sex comb on midleg homolog 1 | |
| AU020998 | 5.5 ± 1.3 | no | no | yes | tissue plasminogen activator | |
| AU018863 | 5.5 ± 0.9 | no | no | yes | Kruppel-like factor 4 | |
| AU022218 | 4.0 ± 0.4 | no | no | yes | protein tyrosine phosphatase 4a1 (also clone ID AW548730) | |
| AW558398 | 2.9 ± 0.3 | yes | no | yes | villin 2 (also clone ID AW552089) | |
| AW546733 | 2.8 ± 0.3 | no | no | no | actin related protein 2/3 complex, subunit 3 (21 kDa) | |
| AW550463 | 2.8 ± 0.2 | no | no | yes | activating transcription factor 4 | |
| AW551165 | 2.7 ± 0.4 | yes | no | no | annexin A2 | |
| AU020799 | 2.6 ± 0.2 | no | no | – | RIKEN cDNA 2410129E14, ortholog to Hs. tubulin b2 | |
| AU023128 | 2.5 ± 0.1 | no | no | yes | protein kinase, cAMP dependent regulatory, type 1α | |
| AW553335 | 0.5 ± 0.1 | no | no | no | ADP-ribosylation-like 2 | |
| AW559016 | 0.4 ± 0.1 | no | no | no | EST (Ug cluster Mm. 5264), ortholg to Hs. FEZ1 | |
| AW546306 | 0.4 ± 0.1 | no | no | no | high mobility group box 2 | |
| AW551831 | 0.4 ± 0.1 | no | no | no | upregulated by 1,25-dihydroxyvitamin D-3 | |
| Growth arrest | ||||||
| AW549049 | 8.8 ± 1.8 | yes | yes | yes | RIKEN cDNA 1190005M04, ortholog to Hs. Serpin 5 | |
| AW557658 | 6.4 ± 0.6 | no | no | yes | MAP kinase-activated protein kinase 2 | |
| AU015509 | 3.7 ± 0.6 | no | no | yes | EST (Ug cluster Mm. 29537), ortholog to Hs. AXUD1 | |
| Cell growth | ||||||
| C87164 | 15.9 ± 0.9 | yes | no | yes | immediate early response 3 | |
| AU023169 | 14.8 ± 5.1 | yes | yes | yes | regulator of G-protein signaling 2 (also clone ID C85919) | |
| AU020132 | 11.1 ± 1.5 | yes | no | yes | ornithine decarboxylase (also clone ID AW537017) | |
| AU015284 | 4.9 ± 0.9 | no | yes | yes | LIM only 1 | |
| AW554898 | 4.5 ± 0.4 | no | no | yes | growth arrest specific 1 | |
| AW554903 | 3.9 ± 0.5 | yes | no | yes | EST (Ug cluster Mm. 26266), ortholog to Hs. protein phosphatase 2, catalytic subunit, α isoform | |
| AW543680 | 3.8 ± 0.5 | no | no | yes | galectin 3 | |
| AU016354 | 0.5 ± 0.1 | no | no | no | protein tyrosine phosphatase 4a3 | |
| AU045362 | 0.5 ± 0.1 | no | no | no | cyclin B2 | |
| C77364 | 0.5 ± 0.1 | no | no | no | ligase I, DNA, ATP-dependent | |
| AW549381 | 0.4 ± 0.1 | no | no | yes | ral guanine nucleotide dissociation stimulator | |
| AW555913 | 0.4 ± 0.1 | no | no | no | mini chromosome maintenance deficient 6 (S. cerevisiae) | |
| Drug resistance | ||||||
| AU016777 | 3.9 ± 1.2 | yes | no | yes | P450 cytochrome oxidoreductase | |
| C77965 | 3.4 ± 0.3 | yes | no | no | fibroblast growth factor regulated protein | |
| AU018999 | 3.1 ± 0.3 | no | no | yes | crystallin, αC | |
| AU018609 | 2.6 ± 0.2 | no | no | yes | ATP-binding cassette, sub-family C, member 3 | |
| AU022813 | 0.5 ± 0.1 | no | no | no | EST (Ug cluster Mm. 22898), ortholog to Hs. mitogen-activated protein kinase 9 | |
| AU020161 | 0.5 ± 0.1 | no | no | no | X-ray repair complementing defective repair in CHO 1 | |
| Intracellular traffic | ||||||
| AW544709 | 4.4 ± 0.8 | yes | no | yes | RIKEN cDNA 2210022F10 ortholog to Hs. EHD4 (also clone ID AU044505) | |
| AU043959 | 3.1 ± 0.3 | no | no | yes | HIV-1 Rev binding protein | |
| AU020150 | 3.0 ± 0.2 | no | no | yes | Mm. clone IMAGE: 3154023, ortholog to Hs. CPSF4 | |
| AU043420 | 0.5 ± 0.1 | no | no | no | Mm. clone IMAGE: 3491260, ortholog to Hs. hook2 protein | |
| Metabolism | ||||||
| AU020528 | 9.3 ± 1.3 | yes | yes | yes | insulin-responsive glucose transporter | |
| AU019144 | 3.8 ± 0.5 | no | yes | yes | EST (Ug cluster Mm. 199789) ortholog to Hs. CITED1 | |
| AU022525 | 2.7 ± 0.3 | no | no | yes | solute carrier family 7, member 8 | |
| AU043052 | 2.7 ± 0.3 | no | no | no | diacylglycerol O-acyltransferase 2 | |
| AW550812 | 2.6 ± 0.1 | no | no | no | aldo-keto reductase family 1, member B1 | |
| AW557657 | 0.5 ± 0.0 | no | no | no | isocitrate dehydrogenase 1 | |
| AU021456 | 0.4 ± 0.2 | no | yes | no | sorbitol dehydrogenase 1 | |
| AW547245 | 0.3 ± 0.1 | no | no | yes | secreted acidic cysteine rich glycoprotein (also clone ID AW536169) | |
| Unknown | ||||||
| AU020865 | 9.4 ± 1.9 | yes | yes | yes | EST (Ug Mm. 26243) | |
| AW553563 | 5.8 ± 0.6 | no | no | yes | RIKEN cDNA 4921531D01 | |
| AU016391 | 4.9 ± 0.8 | yes | no | yes | EST (Ug cluster Mm. 17000) | |
| AU022455 | 4.4 ± 0.6 | no | no | yes | Mm. clone IMAGE: 4215628 | |
| AW550633 | 4.3 ± 0.6 | yes | yes | yes | RIKEN cDNA 2810002E22 | |
| AU018728 | 4.1 ± 0.7 | no | yes | yes | EST (Ug cluster Mm. 26134) | |
| AU019731 | 3.9 ± 0.5 | no | yes | yes | EST (Ug cluster Mm. 173419) | |
| AW554859 | 3.7 ± 0.2 | yes | no | yes | EST (Ug cluster Mm. 40831) | |
| AW555539 | 3.7 ± 0.2 | no | no | yes | RIKEN cDNA 2410019A14 | |
| C86693 | 3.6 ± 0.6 | yes | no | no | DNA segment, Chr 7, ERATO Doi 458 | |
| AU042737 | 3.4 ± 0.4 | no | no | yes | Mm. clone IMAGE: 3586282 | |
| AU019102 | 3.4 ± 0.4 | yes | no | yes | RIKEN cDNA 4930442A21 | |
| AW551294 | 3.3 ± 0.1 | no | yes | yes | RIKEN cDNA 2810428A13 | |
| AU043374 | 3.3 ± 0.4 | no | no | yes | RIKEN cDNA 1110034C02 | |
| AU022753 | 2.8 ± 0.2 | no | no | yes | DNA segment, Chr 17, ERATO Doi 562 | |
| AW543519 | 2.7 ± 0.1 | no | no | yes | EST (Ug cluster Mm. 188460) | |
| AU015210 | 2.6 ± 0.3 | no | no | yes | RIKEN cDNA 2310004N11 | |
| AU024582 | 2.5 ± 0.1 | no | no | yes | EST (Ug cluster Mm. 24260) | |
| AW536239 | 2.4 ± 0.2 | no | no | no | RIKEN cDNA 2610024B07 | |
| AU021752 | 2.3 ± 0.1 | no | no | yes | platelet-activating factor acetylhydrolase, isoform 1β | |
| AA409629 | 0.5 ± 0.1 | no | no | no | EST (Ug cluster Mm. 200884) | |
| AW552690 | 0.5 ± 0.1 | no | no | yes | EST (Ug cluster Mm. 27628) | |
| AW554776 | 0.5 ± 0.0 | no | no | no | RIKEN cDNA 2900070I05 | |
| AU042856 | 0.5 ± 0.1 | no | no | no | RIKEN cDNA 1110068E11 | |
| AW539579 | 0.4 ± 0.1 | no | no | no | RIKEN cDNA 2700059D21 | |
| C86958 | 0.4 ± 0.1 | no | yes | yes | ESTs (Ug cluster Mm. 24807) | |
Direct, a gene is considered to be directly regulated if its regulation by PACAP does not require de novo protein synthesis. AXUD1, axin1 up regulated 1; CITED1, CBP/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1; CPSF4, cleavage and polyadenylation specific factor 4, 30 kDa subunit; EHD4, eps 15 homology domain containing 4; ERK, extracellular regulated kinase; FEZ1, fasciculation and elongation protein zeta 1; Hs., homo sapiens; Mm., mouse musculus; PKA, protein kinase A; PP2CSαI, protein phosphatase 2 catalytic subunit α isoform; Ug, unigene.