Fig. 2. GSK3 binds Nrf1.
(A) HEK293 cells were transfected with vector or Nrfl-V5. After 48 h, whole cell extracts were prepared, and immunoprecipitated with GSK3 antibody followed by immunblotting against V5 and GSK3. (B) HEK293 cells were transfected with vector or GSK3-myc and whole cell extracts were prepared 48 h after and immunoprecipitated with anti-Myc antibody. Immunoprecipitates were then immunoblotted with anti-Myc and anti-Nrf1 antibody.