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. Author manuscript; available in PMC: 2014 Oct 6.
Published in final edited form as: Exp Cell Res. 2013 Apr 23;319(13):1922–1931. doi: 10.1016/j.yexcr.2013.04.013

Fig. 4. GSK3 Phosphorylates Nrf1 in-vitro.

Fig. 4

(A) Purified GST-Nrf1, GST-S350A–Nrf1, GST-Nrf1CPD were subjected to in-vitro ldnase assay with recombinant GSK3β in the presence of γ-32P ATP. Reaction products were separated on SDS-PAGE and subjected to autoradiography (upper panel). As input control, equal amounts of purified fusion products were immunoblotted against Nrf1 (lower panel).