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. Author manuscript; available in PMC: 2014 Oct 6.
Published in final edited form as: Dev Biol. 2013 Jul 30;382(1):136–148. doi: 10.1016/j.ydbio.2013.07.017

Figure 9. Endogenous cytokeratin 5 expression in the E6.5 embryo is observed in the ectoplacental cone and trophectoderm regions in control embryos.

Figure 9

(A and B) Confocal immunofluorescence microscopy of frozen sections of E6.5 embryos. (A) Sagittal section of control E6.5 embryo was immunostained for cytokeratin 5 (K5; red) and stained with DAPI (blue). The white arrowheads identify K5 signal in the ectoplacental cone (EPC) and outer trophectoderm layer. The white dots enclose the embryo and denote the trophectoderm/decidua (D) border. (B) In the absence of primary antibody against cytokeratin 5 no immunostain signal is detected in the E6.5 embryo. Scale bars, 25 μm.