FIG. 1.

ISM1 is selectively expressed in the human body and upregulated in activated CD4⁺ T cells. (A) Mean expression values (y-axis) from microarray data for 105 human tissues are displayed across the x-axis. CNS, central nervous system; PNS, peripheral nervous system; DS, digestive system and oral mucosa; St., muscle, adipose, skin; Va., heart and blood vessels; RS, respiratory system; E, endocrine organs; Ur., kidney, urethra; RT, reproductive tract (male and female); Imm., immune tissues; Leu., peripheral blood cells (monocytes, B and T cells), Em, embryonic (fetal kidney, brain, and placenta). (B) Tissues with the highest ISM1 expression are shown, based on the average signal intensity values of the corresponding probeset (235182_at) from the BIGE database. The MI signal is the average microarray signal from the replicates for each tissue included in the BIGE database. Values reflect (A). (C) Selected tissues were tested to confirm ISM1 expression by qPCR, both in hISM1 and mISM1. (D) mISM1 was detected by western blot in the supernatants of HEK293 cells transfected with the construct pcDNA3.1⁺/mISM1. (E) ISM1 expression was measured by qPCR in resting or activated mouse naive CD4⁺ lymph node T cells, human PBMCs, or Jurkat cells. A representative experiment (out of 3) is shown in (C) and (D). BIGE, body index of gene expression; hISM1, human isthmin 1; mISM1, mouse isthmin 1; MI, mean intensity; PBMCs; qPCR, quantitative real-time polymerase chain reaction.