FIG. 3.

ISM1 expression is associated with Th17 cells and negatively regulated by IFN-γ. (A) Mouse lymph node naive CD4⁺ T cells were cultured under CD4⁺ Th polarizing conditions for 5 days. ISM1 expression was measured under non-restimulated (non-restim) or restimulated (restim) conditions by qPCR. Significance was calculated using the mean and standard deviation of 6 independent experiments. (B) Mouse lymph node naive CD4⁺ T cells were cultured with TGFβ+IL-2 or TGFβ+IL-2+anti-IFN-γ for 4 days. ISM1 expression was measured by qPCR from RNA of non-restimulated or restimulated cells. (C) Analysis of RORγt expression was performed by qPCR as described in (B). Statistics were calculated using Student's t-test from 3 independent experiments. Th, T helper.