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Alkyl labeling of single cysteine mutants and drug effects. The effects of three drugs on EC10 GABA responses were tested before and after butyl methanethiosulfonate (BMTS) labeling, in the absence or presence of GABA, in single cysteine mutants. A. Butanol (33 mM). B. Isoflurane (0.3 mM). C. Flunitrazepam (0.1 μM). (n= 3–5). *p< 0.05, **p< 0.01 versus Cys-less receptor; Student’s t-test.
