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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: J Neurochem. 2013 Oct 28;128(3):363–375. doi: 10.1111/jnc.12476

Table 1.

Changes observed in GABA-induced responses and butanol modulation of GABA-induced responses in selected combinations of GABAAR cysteine mutants after reducing (DTT) and oxidizing (Cu++/Phen) treatment. Change is compared with the control (Cys-less receptor) in equivalent conditions. The color indicates the color of the amino acid in the model representations.

GABAA receptor GABA responsesa Butanol effectb
After DTT After Cu++/Phen After crosslink
 α1β2γ2 Cys-less (control) No change No change No change
Inter-subunit
  graphic file with name nihms532833t1.jpg Decrease No change Increasec
  graphic file with name nihms532833t2.jpg Increase Increase Spontaneous crosslink
  graphic file with name nihms532833t3.jpg No change No change No change
  graphic file with name nihms532833t4.jpg Increase Decrease Spontaneous crosslink
Intra-subunit
  graphic file with name nihms532833t5.jpg Increase Decrease Spontaneous crosslink
  graphic file with name nihms532833t6.jpg No change No change Decrease
   graphic file with name nihms532833t7.jpg Increase Increase Spontaneous crosslink
  graphic file with name nihms532833t8.jpg No change No change Decrease
  graphic file with name nihms532833t9.jpg Decrease No change Spontaneous crosslink
  graphic file with name nihms532833t10.jpg No change No change Decrease
  graphic file with name nihms532833t11.jpg Increase No change Increasec
a

EC50 GABA, except α1(L232C)β2(F289C)γ2 (100 μM) and α1β2(T225C,N265C)γ2 (1 mM)

b

Butanol modulation of EC10 GABA

c

Probably global effect, as it also showed changes in the flunitrazepam effect