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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Cell Microbiol. 2014 Jan 24;16(7):1068–1079. doi: 10.1111/cmi.12260

Figure 5. Constitutively activated Cdc42 inhibits Listeria protrusion formation.

Figure 5

A. Effect of Cdc42V12 on protrusions. Caco-2 BBE1 cells transiently expressing EGFP or YFP-tagged Cdc42V12 were infected with the indicated Listeria strains for 5.5 hours, followed by fixation and immunolabeling. Confocal microscopy analysis was used to quantify efficiencies of protrusion formation (A), or bacterial F-actin recruitment (B), or F-actin tail length (C). Data are mean +/− SEM values from three experiments. Statistical analysis by ANOVA indicated P <0.0001. *, P < 0.05.