FIG. 2.

Dynamic changes in the lesion after moderate clip-compression injury at C6. (A) Representative axial sections through the injury epicenter stained with Luxol fast blue/hematoxylin and eosin at 2, 4, 6, and 8 weeks postinjury. (B) White matter (WM) preservation rostral and caudal to the lesion epicenter. (C) Total percentage of spared WM at 2, 4, 6, and 8 weeks postinjury. (D) Gray matter (GM) preservation rostral and caudal to the lesion epicenter. (E) Injury results in a significant decrease in GM at all time points, as compared to laminectomy-only (L.O.) animals. (F) Percentage of lesional tissue rostral and caudal to the injury epicenter. (G) The volume of lesional tissue decreases as recovery proceeds. None of these decreases were statistically significant. (H) Percentage of cavity volume rostral and caudal to the injury epicenter. (I) The total volume of the cavity increases over time. None of these increases were statistically significant. (B, D, F, and H) Statistical significance was determined by a two-way analysis of variance (ANOVA) with Tukey's post-hoc analysis (p<0.05). Black markers represent values that are significantly different from L.O. Single asterisks represent values that are significantly different from 6 weeks postinjury. Δ represents values that are significantly different from 8 weeks postinjury. (C, E, G, and I) Statistical significance was determined using a one-way ANOVA with Tukey's post-hoc analysis (p<0.05). Single asterisks represent values that are significantly different from L.O. (n=3 in L.O. group; n=4 in each time point postinjury). Data are presented as mean±standard error of the mean. Scale bar=50 μM. Color image is available online at www.liebertpub.com/neu