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. 2014 Mar 17;20(10):790–797. doi: 10.1089/ten.tec.2013.0458

Table 1.

Design of Experiment for Antimicrobial and Cytotoxicity Evaluation of Scaffolds Either Separately or in a Coculture System

  Scaffold Replicates Analyses
Cytotoxicity experiment Pure PLA 3 AlamarBlue assay
  PLA+AgNPs 3 DNA assay
  PLA+AgMPs 3  
Antimicrobial experiment Pure PLA 3 Spectrophotometry
  PLA+AgNPs 3 Plate count
  PLA+AgMPs 3  
Coculture experiment Pure PLA 3 Spectrophotometry
  PLA+AgNPs 3 Plate count
  PLA+AgMPs 3 DNA assay

PLA, polylactic acid; AgMPs, silver microparticles; AgNPs, silver nanoparticles.