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. 2014 Oct 6;9(10):e106625. doi: 10.1371/journal.pone.0106625

Figure 1. Unpredictable chronic mild stress induces the comorbidities of depression-like behavior and hyperhomocysteinemia in rats.

Figure 1

The depression-like behavior of CUMS rats was measured at 2, 4, 6 and 8 weeks. A. The rats were weighed weekly, and the weights are shown. B. A 2-bottle sucrose preference test was used to measure the rats' preferences for sweetened solution over water. Preference was calculated as the percentage of sucrose solution consumed compared with the total fluid intake: %sucrose preference = (sucrose intake/total fluid intake)×100. C. Exploratory behavior was evaluated as described in the Materials and Methods section. D. The immobility time was detected by manual measurements. E. 5-HT level in the hippocampus was detected using liquid chromatography (Waters E2695). F. The total plasma Hcy values were determined by high-performance liquid chromatography (HPLC) with fluorimetric detection and isocratic elution. Values represent the group mean ± structural equation modeling (SEM) (n = 8 rats per group). *P<0.05 compared with control, repeated measures ANOVA followed by Tukey's multiple comparison tests.