Figure 2. Effects on bicalutamide-resistant BicR cell growth by treatment with selected siRNAs that target genes determined by shRNA screening.

Growth inhibition of BicR cells by siRNA targeting RPL31 (siRPL31), HIST1H2BD (siHIST1H2BD), and ADAMTS1 (siADAMTS1) was shown. Cells were transfected with 10 nM siRNA in culture medium. Twelve hours after transfection, cells were then further cultured in medium containing 1 µM bicalutamide. WST-8 cell proliferation assays were performed at the indicated time points after transfection. The absorbance of the wells in the plates was measured using a microplate reader at 450 nm. Data are presented as mean ± s.d. (n = 3; *, P<0.05; **, P<0.01).