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. 2014 Oct 6;9(10):e108743. doi: 10.1371/journal.pone.0108743

Figure 6. p53 partially mediates the function of RPL31 in BicR cells.

Figure 6

(A) Knockdown effects of RPL31 and p53 on MDM2 and p21 mRNA. BicR cells were transfected with siRPL31, sip53, siRPL31 plus sip53, or siLuc. qRT-PCR for RPL31, p53, MDM2, and p21 mRNAwas performed. Experiments were performed in triplicate; mRNA expression is normalized to GAPDH and shown as mean ± s.d. (n = 3; **, P<0.01). (B) sip53 partially cancelled the repression of cell growth induced by siRPL31. BicR cells were transfected with 10 nM each siRPL31, sip53, siRPL31 plus sip53 or siLuc, and cultured with the medium containing 1 µM bicalutamide. WST-8 cell proliferation assay was performed at the indicated time points. The absorbances of the wells in the plates were measured using a microplate reader at a 450 nm. Data are presented as mean ± s.d. (n = 4; **, P<0.01).