Table 1. Summary of purification of A. gorakhpurensis lectin.

Step	Volume (mL)	Total Titre	Total Protein (mg)	Specific Activity (Titre/mg)	Yield (%)	Fold Purification
Crude extract	4.0	128	65.64	1.95	100	1
Affinity purified lectin	2.0	256	7.02	36.46	50	18.6
Ultrafiltrate	1.0	256	6.40	40.00	50	20.5

The lectin was purified by affinity chromatography on mucin-sepharose column and fractions (1.0 mL) were collected. Bound lectin, eluted by EDTA (0.02 M) was recovered in only 2 fractions. Combined fractions were dialysed and assayed for lectin activity and protein content.