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A: Astrocytes were transfected with an siRNA-actin (Control) or siRNA-Nkx6.1 for 6 hours and immunostained with anti-GFAP antibody. Scale bar 100 µm. B: Realtime-PCR analysis of the mRNA levels of Nkx6.1, GFAP, Olig2 and STAT3 in astrocytes after transfection with Control or siRNA-Nkx6.1. The fluorescent output signal of each gene was measured and normalized to the corresponding control. (n = 3. *P<0.05. **P<0.01).
