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. 2014 Oct 6;9(10):e109171. doi: 10.1371/journal.pone.0109171

Figure 7. Abnormal Slit1 expression in spinal cords at embryo stages of Nkx6.1 mutant.

Figure 7

Spinal cord sections from E12.5 to E18.5 embryos were subjected to ISH with Slit1 riboprobe in WT and Nkx6.1−/− mice. Slit1 expression in the ventral white matter was significantly reduced at E16.5. At E18.5, Slit1 expression was observed in the ventral astrocyte subtypes 2 and 3 (VA2 and VA3) in wild-type embryos, but only in VA3 astrocytes in Nkx6.1 mutants (outlined by dashed lines in G- H). Scale bars: 100 µm.