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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Acta Biomater. 2014 Aug 10;10(11):4759–4767. doi: 10.1016/j.actbio.2014.08.003

Figure 5.

Figure 5

Detection of I-Ad dAPCs in draining lymph nodes. αI-Ad was administrated at the graft bed with the self-assembling components (“αI-Ad membrane”) or PBS (“αI-Ad IgG, local”) prior to the skin graft. Single cell suspensions enriched for APCs were prepared from draining lymph nodes of recipient C57BL/6 mice. Cells were stained with Allophycocyanin conjugated anti-mouse MHC-II I-Ad antibody, and flow cytometry was used to probe the frequency of I-Ad dAPCs. (a) Representative dAPCs frequency on day 2. Student’s t test was used to confirm the statistical difference on day 1(b, n=4, ns: not significant), day 2 (c, n=3-, *: p < 0.05), and day 3 (d, n=3, ns: not significant).