Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 7;55(3):467–481. doi: 10.1016/j.molcel.2014.05.031

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Published by Elsevier Inc.

This work is licensed under a Creative Commons Attribution 3.0 Unported License.

PMC Copyright notice

Analysis of the Effect of rNrd1-Nab3 on the Polyadenylation Activity of rTrf4-rAir2 In Vitro

(A) Polyadenylation assays with recombinant Trf4-Air2 in the absence or in the presence of recombinant Nrd1-Nab3. Left: PAGE analysis of polyadenylated species at 10, 20, and 30 min reaction time. The position of the substrate and the number of added As is indicated. Right: lane scans of the gel shown on the left.

(B) Polyadenylation assays as in (A) to individually assess the role of rNrd1, rNab3, and the Nrd1 CID domain in stimulating polyadenylation by rTrf4-rAir2. Reactions were performed with rTrf4-rAir2 in the presence of the indicated proteins or protein complex.