Fig. 2.
Immunocytochemical detection of megalin in ultrathin cryosections from mouse RPE and NPCBE cells. Immuno-gold labeling (arrow heads) shows that megalin localizes to vesicles of the endocytic apparatus in the apical (a) as well as the basolateral (b) regions of RPE cells. Megalin is furthermore detected in the apical membrane as well as the apical endocytic apparatus of NPCBE cells (c). POS photoreceptor outer segment, M melanosome, B basement membrane, N nucleus. Scale bar 0.5 µm