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Effect of CrTX on the expressions of PCNA and cleaved caspase-3 in SK-MES-1 cells. SK-MES-1 cells were treated with 50 μg/mL CrTX for 24, 48, and 72 h. Cell lysates were prepared and protein levels of PCNA and caspase-3 were determined with Western blot analysis. (A) The expression of PCNA and quantification. (B) The expression of cleaved caspase-3 and quantification. β-Actin was used for normalization and verification of protein loading. n=3. Mean±SD. bP<0.05, cP<0.01 vs controls.
