Effects of CrTX on the expression of LC3, Beclin 1, and p62 in SK-MES-1 cells. SK-MES-1 cells were treated with 50 μg/mL CrTX for 24, 48, and 72 h. Cell lysates were prepared and protein levels of LC3, Beclin 1, and p62 were determined with Western blot analysis. (A) The expression of LC3 and quantification. (B) The expression of Beclin 1 and quantification. (C) The expression of p62 and quantification. β-Actin was used for normalization and verification of protein loading. n=3. Mean±SD. bP<0.05, cP<0.01 vs controls.