Figure 6.

Effects of p38MAPK on CrTX-induced cytotoxicity in SK-MES-1 cells. SK-MES-1 cells were treated with 50 μg/mL CrTX for 24, 48, and 72 h. Cell lysates were prepared and protein levels of p-p38 and p38 were determined by Western blotting analysis. SK-MES-1 cells were pretreated with or without SB (10 or 20 μmol/L, 1 h), followed by 50 μg/mL of CrTX for 48 h, and then cell viability was determined by MTT assay. (A) The expression of p-p38 and quantification after CrTX induction at indicated time. β-Actin was used for normalization and verification of protein loading. (B) The expression of p38 and quantification. (C) Effect of SB203580 on the cytotoxicity of CrTX. n=3. Mean±SD. ^bP<0.05, ^cP<0.01 vs controls.