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. 2014 Aug 18;35(10):1323–1332. doi: 10.1038/aps.2014.62

Figure 8.

Figure 8

Effect of SB on CrTX-induced apoptosis of SK-MES-1 cells. SK-MES-1 cells were pretreated with or without SB (10 μmol/L, 1 h), followed by 50 μg/mL of CrTX for 48 h. (A) Apoptosis of SK-MES-1 cells. Cells were collected and stained with Annexin V-FITC/PI, and then subjected to flow cytometry to detect apoptotic cells of SK-MES-1 cells. (B) The expression of cleaved caspase-3 was detected by Western blot analysis. β-Actin was used for normalization and verification of protein loading. n=3. Mean±SD. bP<0.05, cP<0.01 vs controls.