Figure 2.

Telekin-induced cell-cycle arrest at the G₂/M phase. HepG2 cells were treated for 24 h with various concentrations of telekin (0–15 μmol/L) or with 15 μmol/L telekin for various times (0–24 h). (A) The cell cycle distribution was measured using flow cytometry. (B) The histograms indicate the cell cycle distribution in HepG2 cells treated with telekin. ^bP<0.05, ^cP<0.01 vs 0 μmol/L telekin group. (C) HepG2 cells were stained with DAPI to determine the morphology of nucleus. Treatment with telekin resulted in nuclei unable to segregate after DNA replication. DAPI: 4′,6-diamidino-2-phenylindole.