Figure 5.

Eliminating ROS blocked telekin-mediated cell cycle arrest. HepG2 cells were pretreated with 0, 2.5, 5, or 10 mmol/L NAC and treated with 15 μmol/L telekin or were pretreated with 5 mmol/L NAC for 1 h and then treated with 0, 5, 10, or 15 μmol/L telekin for 24 h. (A) NAC treatment decreased the ROS generation significantly. ^bP<0.05, ^cP<0.01 vs 0 μmol/L telekin+5 mmol/L NAC group. (B) NAC treatment decreased the percentage of cells in the G₂/M phase. The percentage of cells in the G₂/M phase decreased from 50.88% to 18.7% (5 mmol/L NAC). (C) The corresponding histograms are presented below. ^bP<0.05, ^cP<0.01 vs 15 μmol/L telekin+0 mmol/L NAC group, or 0 μmol/L telekin+5 mmol/L NAC group.