Figure 6.

Inhibition of ROS reversed the changes of some major proteins. HepG2 cells were pretreated with NAC (0, 2.5, 5, and 10 mmol/L) and then treated with 15 μmol/L telekin for 24 h. Protein expression was determined by Western blotting. (A) As shown, pretreatment of cells with NAC decreased the expression of phosphor-p38 and phospho-MAPKAPK-2 and increased the expression of Cdc25A and Cdc2 compared with the cells treated with telekin only. (B) The corresponding histograms quantified by densitometry are expressed on the right side. ^bP<0.05, ^cP<0.01 vs 15 μmol/L telekin+0 mmol/L NAC group.